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. 2022 Nov 15;66:101637. doi: 10.1016/j.molmet.2022.101637

Figure 7.

Figure 7

5-lipoxygenase derived lipid mediators contribute to exercise-induced mitochondrial function via AMPK activation. (A) Flow cytometric analysis (MFI) of intracellular AMPKα1(Thr172) phosphorylation in PMs isolated from WT and Alox5−/− mice following exercise. (B) FlowJo V10.8.1 generated t-SNE map (B,C) depicted in a 2D dimensionally reduced dataspace showing all events combined from each experimental groups. The color axis overlay in (B) depicts metacluster populations identified using FlowSOM, an unbiased machine learning clustering algorithm. (C) The percent frequency of each FlowSOM population (highlighted in red) for all samples combined, is denoted atop of the respective t-SNE map. (D) The fluorescence distribution of each surface marker for the respective FlowSOM metacluster population is shown in red (vs the distribution for all cells shown in grey) in the histogram overlays. (E) MFI of TMRM for each FlowSOM metacluster population from WT and Alox5−/− mice following exercise. Data expressed as mean ± SEM; n = 5 (A and E); ∗P < 0.05; two-tailed Student's t-test.