Table 1.

Metal choices of RNases H.

RNase H	Substrate	Metal preference	Source
Mycobacterium smegmatis RNase HI	RNA/DNA		Richa Gupta et al. (2017)
Escherichia coli RNase HI	RNA/DNA		Uchiyama et al. (1994)
Shewanella sp. SIB1 RNase HII	RNA/DNA		Chon et al. (2006b)
Chlamydia pneumonia RNase HII	RNA/DNA		Pei et al. (2005)
Archaeoglobus fulgidus RNase HII	RNA/DNA		Chai et al. (2001)
Pyrococcus kodakaraensis KOD1 RNase HII	RNA/DNA		Haruki et al. (1998)
Aeropyrum pernix RNase HII	RNA/DNA		Hou et al. (2012a)
Bacillus subtilis RNase HII	Dr4D/DNA		Randall et al. (2018)
	Dr1D/DNA
Bacillus subtilis RNase HIII	RNA/DNA		Randall et al. (2018)
	Dr4D/DNA
	Dr1D/DNA

The optimal metal cofactor is in red; edges represent different levels of activity dependent on metal ions. Dr₁D/DNA is the substrate of dsDNA that contains a single ribonucleotide. Dr₄D/DNA is dsDNA with four embedded ribonucleotides.