FIG. 4.

Immunoglobulin-binding reactivity of surface proteins expressed by representative small and large colonies. Single-colony isolates of blood-passaged strain 64/bp9 demonstrating either the small- or the large-colony morphology were grown to stationary phase in THB. Surface proteins following extraction with CNBr were separated by SDS-PAGE, electroblotted to nitrocellulose, and probed as indicated. A representative extract from the parent isolate 64p and an extract from the isolate passaged 14 times in mice (64/14) are included for reference. Blots were developed by an ECL procedure and exposed to Kodak XAR film for 30 to 60 s.