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. 2022 Nov 21;13:991320. doi: 10.3389/fphys.2022.991320

TABLE 1.

Composition and calculated nutrient analysis of basal diets, source and dose of digestible TSAA and supplemental met in experiment #1.

Ingredient Starter, % Grower, %
Corn 52.65 55.53
Soybean meal 34.23 28.39
DDGS 5.00 7.50
Meat and bone meal 3.00 3.00
Choice white grease 2.55 3.27
Limestone 0.93 0.94
Salt 0.42 0.41
Dicalcium phosphate 0.31 0.09
L-Lysine HCl 78% 0.14 0.15
Threonine 0.10 0.09
Choline Cl-60% 0.05 0.05
Vitamin premix 0.05 0.05
Phytase 0.005 0.005
Oro Glo 20 (pigment) 0.10 0.10
Trace mineral premix a , b , c 0.20 0.20
 Methionine 0.27 0.23
 Total 100 100
Calculated nutrient profile
 ME, kcal/kg 3044 3126
 Crude protein, % 23.23 21.32
 Lys, digestible% 1.25 1.12
 Met, digestible% 0.35 0.34
 TSAA, digestible% 0.70 0.66
 Thr, digestible% 0.88 0.80
 Arg, digestible% 1.43 1.28
 Val, digestible% 1.04 0.96
 Trp, digestible% 0.25 0.22
 Ile, digestible% 0.95 0.86
 Ca, % 0.95 0.89
 P, available% 0.50 0.46
 P, total% 0.79 0.73
 Sodium% 0.20 0.20
Analyzed proximate
 Protein, % 23.50 21.54
 Dry matter, % 88.58 87.29
 Ash, % 5.43 5.00
 Fat, % 5.11 6.04
 Fiber, % 2.99 2.59
Digestible TSAA and supplemental Met source and dose
 5% lower dig. TSAA 0.89 0.82
 Suppl. DL-Met, % 0.19 0.16
 Suppl. HMTBa, % 0.22 0.18
 5% higher dig. TSAA 0.98 0.91
 Suppl. DL-Met, % 0.29 0.25
 Suppl. HMTBa, % 0.32 0.28
a

Trace mineral premix were included at 0.2%.

b

Final concentration of minerals in diets supplemented with ITM: zinc sulfate monohydrate, 80 mg/kg; Copper Sulfate, 20 mg/kg; manganese sulfate monohydrate, 100 mg/kg; sodium selenite, 0.3 mg/kg; ferrous sulfate monohydrate, 80 mg/kg; calcium iodate, 1.25 mg/kg.

c

Final concentration of minerals in diets supplemented with MMHAC: Zn-methionine hydroxy-analogue (Zn-MHAC), 40 mg/kg; Cu-MHAC, 10 mg/kg; Mn-MHAC, 50 mg/kg; sodium selenite, 0.3 mg/kg; ferrous sulfate monohydrate, 80 mg/kg; calcium iodate, 1.25 mg/kg.