Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Oligonucleotide set designed by three algorithms. Compared to other algorithms, the depth-first search algorithm does not surpass the integrated algorithm, but it takes less time to process. For a fair comparison, the algorithms were tested under the same conditions.
| Algorithm | Gene | ||
|---|---|---|---|
| S100A4 (752 bp) overlap Tm std | PKB2 (1446 bp) overlap Tm std | GFPuv (760 bp) overlap Tm std | |
| TmPrime | 1.14 | 1.23 | 0.93 |
| Depth-first search | 0.84 | 0.52 | 0.40 |
| Integrated algorithm (gapped) | 0.27 | 0.49 | 0.17 |
