FIGURE 10.

Direct comparison of internalization efficiencies for five 550-labeled MBP-EP-CBS cargos: MBP-CBS, MBP-AUR, MBP-GALA, MBP-LAH4, and MBP-HA2. Cargo alone and adaptor/cargo complexes (1 µM) were internalized in growth media for 1 h prior to washing (PBS x2) and imaging in media containing NucBlue. Internalization of the 5 substrates was concurrently analyzed in (A) with no adaptor present, in (B) with TAT-CaM and in (C) with TAT-LAH4-CaM. The individual experiments represented by each column were independently adjusted to accent pattern differences within each column (for direct comparison of adaptors see Figures 7–9).