FIGURE 2.

Internalization of Cas9 bound to TAT-CaM occurs readily at very low concentration. Cas9-NLS bound to equimolar TAT-CaM at 100 nM was incubated with BHK cells in DMEM with 10% FBS for 1 h prior to PBS treatment, washing and imaging. Panels show (A) 550-labeled TAT-CaM, (B) 488-labeled Cas9-NLS and (C) Composite including NucBlue. In (D) the enlarged inset (Figure 2D) shows Cas 9-NLS and TAT-CaM are often in separate but juxtaposed endosomes.