FIGURE 3.

Specific and nonspecific Cas9 internalization with the TAT-CaM. BHK cells in DMEM with 10% FBS were treated for 1 h with Cas9-NLS in the absence (top) or presence (bottom) of excess TAT-CaM prior to washing (PBS x3) and imaging. Cas9-NLS at concentrations >50 nM showed no increase in internalization with inclusion of TAT-CaM as shown in examples (A) 500 nM and (B) 50 nM; however, at low Cas9-NLS concentrations including (C) 10 nM, TAT-CaM specifically increased internalization.