FIGURE 5.

TAT-CaM specifically induces MBP-CBS internalization but requires µm concentrations. (A) Internalization of MBP-CBS alone (left panels), with equimolar with TAT-CaM (center panels) or with “TAT-less” CaM control (right panels) following 1 h incubation with BHK cells in growth media. Cells were washed (PBS x2) and imaged immediately (top panels) or later (bottom panels) in imaging media with NucBlue. (B) Internalization of equimolar MBP-CBS and TAT-CaM at 4 concentrations following a 2 h incubation, washing (PBS x2) and imaging in media with NucBlue.