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. 2022 Nov 21;13:1070464. doi: 10.3389/fphar.2022.1070464

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Copyright © 2022 Morris, Snipes, Hill, Woods, Mbugua, Wade and McMurry.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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550- MBP-CBS and 550- MBP-GALA internalized with adaptor set including controls. Internalization of equimolar adaptor/cargo complexes (1 µM) in growth media were analyzed as sets of four conditions, each containing the no adaptor cargo as reference. All complexes were internalized for 1 h prior to washing (PBS x2) and imaging in media containing NucBlue. Internalization of CBS-MBP in the upper panel (A) used: no adaptor, TAT-CaM, TAT-AUR-CaM, or TAT-GALA-C and in the lower panel (B) used: no adaptor, “TAT-less” CaM-Ctrl, TAT-LAH4-CaM, and TAT-HA2-CaM. Internalization of MBP-GALA used the same adaptor sets in the upper (C) and lower (D) panels. For presentation, intensities within each set were adjusted identically: however, for these cargos no-adaptor reference are not presented at equal intensity in upper and lower panels to avoid oversaturating internalization with TAT-LAH4-CaM.