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. 2022 Nov 21;13:1070464. doi: 10.3389/fphar.2022.1070464

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Copyright © 2022 Morris, Snipes, Hill, Woods, Mbugua, Wade and McMurry.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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550-MBP-AUR and 550-MBP-HA2 internalized with adaptor set including controls as in prior 2 figures. Equimolar adaptor/cargo complexes (1 µM) were internalized in growth media for 1 h prior to washing (PBSx2) and imaging in media containing NucBlue. Internalization of MBP-AUR and MBP-HA2 with the adaptors as above in both the upper panels (A,C) and the lower panels (B,D). For presentation, intensities within each set adjusted identically but with the no-adaptor reference at approximately equal intensity for all panels.