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. 2022 Nov 21;13:1077328. doi: 10.3389/fpls.2022.1077328

Figure 6.

Figure 6

Validation of LAMP assay with S. oryzae infected paddy and weed samples. Bright sky blue colour represents positive reaction while the violet colour indicates the negative reaction. Total DNA isolated from fungi was used as the positive control. Upper panel in the figure shows agarose gel electrophoresis results whereas lower panel shows reaction tubes for colorimetric detection. M- 100bp Ladder; Lane 1: Oryza sativa; Lane 2: Echinchloa crusgalli: Lane 3: Cyperus difformis; Lane 4: Eleusine indica; Lane 5: Dactyloctenium aegyptium; Lane 6: Cyperus iris; Lane 7: Eragrostis amabilis; Lane 8: Leersia hexandra; Lane 9: Phalaris minor; Lane 10: Brachiaria mutica; Lane 11: Sarocladium oryzae isolate OM841519 (Positive control); Lane 12: Nuclease free water.