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. 2022 Nov 9;298(12):102686. doi: 10.1016/j.jbc.2022.102686

Figure 8.

Figure 8

Direct effects of OSM on skeletal muscle macrophages.A, expression of mRNA of M1 (iNOS and TNF-α) and M2 markers (arginase-1 [Arg1] and IL-10) in the skeletal muscle macrophages isolated from WT and OSM−/− mice at 24 h after the exercise. B–D, effects of OSM on the expression of mRNA related to phenotypes of macrophage (B) and chemokines (C and D) in the skeletal muscle macrophages. Macrophages were isolated from skeletal muscle and then stimulated with OSM (50 ng/ml) for 1 or 2 h (B and C). Macrophages isolated from skeletal muscles were incubated with WP1066 (1 μM) for 1 h prior to OSM treatment (50 ng/ml, 1 h) (D). Data are expressed as mean ± SD; n = 4 to 5 per group. ∗p < 0.05 and ∗∗p < 0.01. Student’s t test (A); two-way ANOVA followed by Tukey’s post hoc test (B–D). CCL, CC chemokine ligand; CXCL1, CXC chemokine ligand 1; IL, interleukin; iNOS, inducible nitric oxide synthase; OSM, oncostatin M; TNF-α, tumor necrosis factor alpha.