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. 2022 Nov 9;8(1):101122. doi: 10.1016/j.adro.2022.101122

Figure 1.

Figure 1

Effect of combining radiation therapy (RT) with CPI-613 on the viability of pancreatic ductal adenocarcinoma (PDAC) cells and the cellular targets of CPI-613. (A) Representative photomicrographs of cells subjected to the indicated doses of radiation and 300 μM CPI-613 for 72 hours were captured at 10 × magnification on an inverted light microscope. (B) Cell viability was determined by trypan blue dye exclusion assay at the end of the experiment and plotted as average number of viable cells ± standard error of the mean (SEM) of 2 replicates. *P < .05 compared with control. #P < .05 to 2 Gy RT alone. ϮP < .05 compared with CPI-613 alone. (C) Protein expression of pyruvate dehydrogenase (PDH) and 2-oxoglutarate dehydrogenase (OGDH) (cellular targets of CPI-613) in MiaPaCa-2 cells irradiated with 2 or 10 Gy radiation and treated with 300 μM of CPI-613 for 72 hours was analyzed by Western blotting.