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. 2022 Nov 9;8(1):101122. doi: 10.1016/j.adro.2022.101122

Figure 4.

Figure 4

Effect of combining radiation therapy (RT)–CPI-613 with chemotherapeutic drugs. (A) MiaPaCa-2 cells were treated with either gemcitabine (Gem; 90 nM) or 5-fluorouracil (5-FU; 12 μM) in the presence of CPI-613 (300 μM) followed by exposure to 2 Gy RT. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed after 72 hours of treatment. Cell viability (% of control) was plotted as an average of 6 replicates ± standard error of the mean (SEM). Combination index (CI) was calculated using CalcuSyn software. CI < 1 indicates synergistic effect. **P < .01. (B) MiaPaCa-2 cells were seeded in ultralow attachment plates and later treated with Gem (100 nM) or CPI-613 (300 μM) either as single agents or in combination followed by irradiation with 2 Gy radiation. Each treatment was performed in triplicate. After 1 week, spheroids were observed under an inverted light microscope, and their images were captured at 10 × magnification.