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. 2022 Nov 21;10:984375. doi: 10.3389/fcell.2022.984375

FIGURE 2.

FIGURE 2

Morphine-ADEVs induce pericyte migration and loss in vitro. (A) The migration of pericytes that responded to morphine was measured by transwell migration assay. Migrated pericytes were stained with crystal violet. Scale bar = 20 µm. Quantification of migrated pericytes is shown on the right. N = 9 (B)The migration of pericytes responded to morphine-ADEVs was measured by transwell migration assay. Migrated pericytes were stained with crystal violet. Scale bar = 20 µm. Quantification of migrated pericytes is shown on the right. N = 9 (C) Migration of pericyte responded to morphine-ADEVs was measured by the scratch assay. The representative images of pericyte stained by crystal violet and then analyzed by ImageJ software. Scale bars, 100 μm. N = 9 (D) Loss of pericyte was measured in the 3D pericyte (green)-endothelia (Red) coculture model. The coverage of pericytes was analyzed by ImageJ. Scale bars, 50 μm. N = 9 All data are presented as mean ± SD of 3 individual experiments, *p < 0.05,**p < 0.01, ***p < 0.001 vs. control group using one-way ANOVA analysis.