FIGURE 7.

Overexpression of PTEN attenuates morphine-ADEV-mediated pericyte migration. (A–C) Pericytes were transfected with either control vector or PTEN overexpression plasmid (PTEN OE) for 24 h, then exposed to either control-ADEVs or morphine-ADEVs. Then the migration of pericyte was measured by transwell migration assay (A). The representative images of migratory pericytes stained with crystal violet. Scale bar = 100 µm. The migration of pericyte was measured by the scratch assay (B). The representative images of pericytes stained with crystal violet and then the representative images were analyzed by ImageJ software. Scale bars, 100 μm. Loss of pericyte was measured by 3D pericyte (green)-endothelia (Red) coculture model (C), and then the representative images were analyzed by ImageJ. Scale bars, 50 μm. All data are presented as mean ± SD, *p < 0.05, ***p < 0.01 vs. control group (Vector-CTL-ADEVs), #p < 0.05, ##p < 0.01 vs. Vector-morphine-ADEVs group using one-way ANOVA analysis.