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. Author manuscript; available in PMC: 2023 Apr 5.
Published in final edited form as: Cancer Discov. 2022 Oct 5;12(10):2308–2329. doi: 10.1158/2159-8290.CD-21-0925

Figure 6. Novel electroporation-derived ccRCC syngeneic model is metastatic and transcriptomically resembles human stromal/proliferative ccRCC molecular subtype.

Figure 6.

(A) Schematic of ccRCC syngeneic cell line development using electroporation of somatic tissues. Photo (B) and H&E (C) and IHC (D) of the parental kidney tumor and surrounding normal kidney. (E) Western blot of the EP-derived LVRCC67 cell line, RenCa and NIH3T3 cells are used as controls. H&E of lung (F) or liver (G) metastatic nodules following subcutaneous injection of LVRCC67 cells into WT C57Bl/6 mice. (H) Heatmap of ssGSEA scores in LVRCC67 tumors compared with normal kidney cortex samples from WT C57Bl/6 mice. Red arrows indicate tumor area. Scale bar 100μm. Schematic was made with BioRender.