TABLE 3.
Protective efficacies of different ST-CF and ESAT-6 subunit vaccines
| Vaccinea | Log10 resistanceb
|
||
|---|---|---|---|
| Spleen | Liver | Lung | |
| Expt 1 | |||
| ST-CF + MPL | 0.10 (±0.08) | 0.07 (±0.10) | |
| ST-CF + DDA | 0.69 (±0.10)* | 1.09 (±0.04)* | |
| ST-CF + DDA–MPL | 0.93 (±0.11)* | 1.30 (±0.11)* | |
| BCG | 1.29 (±0.09)* | 1.33 (±0.10)* | |
| Expt 2 | |||
| MPL | <0.05 | <0.05 | |
| DDA | <0.05 | <0.05 | |
| DDA + MPL | <0.05 | 0.10 (±0.11) | |
| ESAT-6 + MPL | <0.05 | 0.21 (±0.12) | |
| ESAT-6 + DDA | 0.16 (±0.15) | 0.14 (±0.10) | |
| ESAT-6 + DDA–MPL | 0.67 (±0.17)* | 0.85 (±0.13)* | |
| BCG | 1.25 (±0.19)* | 0.70 (±0.15)* | |
| Expt 3 | |||
| DDA + MPL | <0.05 | <0.05 | |
| ESAT-6 + DDA–MPL | 0.89 (±0.32)* | 0.50 (±0.09)* | |
| BCG | 0.94 (±0.24)* | 0.46 (±0.09)* | |
a
Mice were vaccinated once with BCG or three times at 2-week intervals with the subunit vaccines.
b
Expressed as log10 reductions in the bacterial loads measured in the spleen and lung 6 weeks after aerosol challenge (experiments 2 and 3) or in the spleen and liver 2 weeks after i.v. (experiment 1). TB infection and compared to those in the adjuvant controls. Data are means based on duplicate analysis of five animals in each group. Asterisks, bacterial loads significantly different from those in control mice.