Figure 2.

Initial alignment of the first closed RyR2 subunit with the first open RyR2 subunit. An alignment from the initial models of the first subunit in the closed 4C RyR2 model system (tan) and the first subunit in the open 4O RyR2 model system (light blue) before minimization and MD simulations were carried out. The S4S5L (residues 4746–4766) is highlighted for the closed RyR2 model system (blue) and for the open RyR2 model system (red). The alignment of the two subunits was performed using UCSF Chimera 1.15, while the images were visualized using UCSF Chimera X 1.4.