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. Author manuscript; available in PMC: 2022 Dec 5.
Published in final edited form as: Cell Rep. 2022 Oct 25;41(4):111529. doi: 10.1016/j.celrep.2022.111529

KEY RESOURCES TABLE.

REAGENT or RESOURCE SOURCE IDENTIFIER
Bacterial and virus strains
E. coli strain BL21 (DE3) Rosetta2 Millipore Cat#71400
Biological samples
hP13 ZnF5-WWE1-WWE2 (residues 507–699) WT protein This paper N/A
mP13 ZnF5-WWE1-WWE2 (residues 476–673) WT protein This paper N/A
Chemicals, peptides, and recombinant proteins
PARP1 3rd Zinc finger (Langelier et al., 2008) N/A
WWE of RNF146-GST tagged (Wang et al., 2012) N/A
ADPr Sigma-Aldrich Cat#A0752
ATP Sigma-Aldrich Cat#A2383
Iso-ADPr Dr. Wenqing Xu, University of Washington N/A
18-mer PAR FAM labeled on 2′ end (PAR 2′ FAM) (Ando et al., 2019) N/A
18-mer PAR FAM labeled on 1″ end (PAR 1″ FAM, AMP-(ADPr)18) (Abraham et al., 2020) N/A
20-mer PAR FAM labeled on 1″ end (PAR 1″ FAM, AMP-(ADPr)20) (Abraham et al., 2020) N/A
Unlabeled PAR, AMP-(ADPr)1–9 This paper N/A
ATP-FAM labeled on gamma-phosphate Jena-Bioscience Cat#NU-833-5FM
Deposited data
mP13 ZnF5-WWE1-WWE2 (residues 476–673): ATP This paper PDB: 7SZ2
mP13 ZnF5-WWE1-WWE2 (residues 476–673): ADPr This paper PDB: 7SZ3
Oligonucleotides
RNA-FAM with a 6-FAM group on the 5′ end IDT N/A
RNA-NB-FAM with a 6-FAM group on the 5′ end IDT N/A
Recombinant DNA
hP13 ZnF5-WWE1-WWE2 (residues 507–699) WT- Gb1 tag This paper N/A
hP13 ZnF5-WWE1-WWE2 (residues 507–699) W611A-Gb1 tag This paper N/A
hP13 ZnF5-WWE1-WWE2 (residues 507–699) R577A-Gb1 tag This paper N/A
hP13 ZnF5-WWE1-WWE2 (residues 507–699) R684A-Gb1 tag This paper N/A
mP13 ZnF5-WWE1-WWE2 (residues 476–673)-SMT This paper N/A
NZAP, hP13 zinc finger 1–4 (residues 1–225) This paper N/A
WWE of RNF146-GST tagged (Wang et al., 2012) N/A
Software and algorithms
ASTRA 6.1.6.5 Wyatt technology https://www.wyatt.com/products/software/astra.html
FOXS (Schneidman-Duhovny et al., 2013) https://modbase.compbio.ucsf.edu/foxs
CRYSOL (Svergun et al., 1995) https://www.embl-hamburg.de/biosaxs/manuals/crysol.html
BioXTAS RAW (Hopkins et al., 2017) https://bioxtas-raw.readthedocs.io/
DENSS (Grant, 2018) https://www.tdgrant.com/denss/
XDS (Kabsch, 2010) https://xds.mr.mpg.de
COOT (Emsley et al., 2010) https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
PHENIX (Adams et al., 2010) http://www.phenix-online.org
MATLAB MathWorks https://www.mathworks.com/products/matlab.htm