TABLE 1.
L. major-βGAL induces CD4 L. major- and β-GAL-specific T cells
| Antigenic stimulus | % Positive cellsa
|
||||
|---|---|---|---|---|---|
| CD4 | CD8 | T-cell receptor
|
B220 | ||
| αβ | γδ | ||||
| L. major | 82 | 12 | 76 | 3 | 4 |
| β-GAL | 80 | 15 | 78 | 2 | 3 |
a
Techniques used for obtaining T-cell lines and fluorescence-activated cell sorting analysis of the surface phenotype of the cells are given in Materials and Methods. The experiments were performed with LNC originally obtained from the lymph nodes draining lesions of L. major-βGAL in BALB/c mice infected with the parasite for 3 weeks. The data are representative of three independent experiments.