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. 2022 Dec 5;15:128. doi: 10.1186/s13048-022-01064-3

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Differential gene expression and RNA quality of semi-automatically and manually vitrified oocytes. A Comparison of mean medTIN between semi-automated and manual vitrification groups. Significance was assessed using a linear mixed model with random effects. B Volcano plot representation of the differential expression analysis with semi-automated vitrification compared with manual vitrification (Semi-automated minus Manual). Each point represents one of the genes expressed with log2(FC) on the x-axis and –log10 of the unadjusted p-value calculated with DESeq2 on the y-axis