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. 2000 Feb;68(2):824–831. doi: 10.1128/iai.68.2.824-831.2000

FIG. 1.

FIG. 1

Orientation and nucleotide position of the different primers used for PCR and sequencing of the central regions of the tprK ORF as well as the complete ORF and its flanking regions. Solid lines represent the ORF of the tprK gene. Primer positions are as follows: F5-S, base positions 975967 through 975987 upstream of the tprK start codon; FW-S, base positions 975809 to 975833; 9V-S, base positions 975629 to 975652 in the tprK ORF; tprK-S, base positions 975162 to 975187 in the tprK ORF; tprK-As, base positions 974778 to 974800 in the tprK ORF; 9V-As, base positions 974708 to 974730 in the tprK ORF; FW-As, base positions 974319 to 974341 in the tprK ORF; and F3-As, base positions 973922 to 973943 downstream of the tprK stop codon.