FIG. 3.

Alignment of the TprK internal region predicted amino acid sequences of T. pallidum isolates obtained with the tprK-S and tprK-As primers showing highly conserved regions and three variable domains. Shaded areas indicate sequence identity, and broken lines indicate gaps in the alignment. NicholsGen, Nichols strain sequence as reported in the T. pallidum genome sequence; NicholsSea, sequence of the Nichols strain maintained in our laboratory. The street isolates shown in this alignment are Bal 7, Bal 73-1, and Sea 81-4 listed in Table 1. The sequences in Fig. 3 to 5 are indicated by isolate name, followed by the clone designation. For example, Bal 73-1.306 is the sequence from clone 306 of the Bal 73-1 isolate.