REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CD3	BioLegend	OKT3; Cat#317328; RRID: AB_2562907
CD4	BioLegend	RM4-5; Cat#100526; RRID: AB_312727
CD19	BioLegend	SJ25C1; Cat#363024; RRID: AB_2564253
CD16	BioLegend	3G8; Cat#302044; RRID: AB_2563802
CD14	BioLegend	M5E2; Cat#301838; RRID: AB_2562909
IgG	BD Biociences	G18-145; Cat#555786; RRID: AB_396121
Goat F(ab')2 Anti-Mouse IgG(H + L), Human ads-AP	Bioconcept	Cat#1032-04; RRID: AB_2794320
Biological samples
Donors’ PBMCs	This study
Donors’ sera	This study
Chemicals, peptides, and recombinant proteins
R848 (Resiquimod)	InvivoGen	Cat#tlrl-r848-5
Recombinant human IL-2	ImmunoTools	Cat#11340027
RBD, mouse Fc Tag	Sino Biological	Cat#40592-V05H
pNPP	Sigma-Aldrich	Cat#71768-25G
SARS-CoV-2 S2	The Native Antigen Company	Cat#REC31807-500
SARS-CoV-2 S1	The Native Antigen Company	Cat#40150-V08B1
SARS-CoV-2 N	The Native Antigen Company	Cat#REC31812
HCoV-OC43 N	The Native Antigen Company	Cat#REC31857
SARS-CoV-2 NTD	McCallum et al.13
SARS-CoV-2 S	Walls et al.28,47
SARS-CoV S	Walls et al.48
HCoV-OC43 S	Pinto et al.49
MERS-CoV S	Walls et al.48
HCoV-HKU1 S	Pinto et al.49
SARS-CoV-2 RBD	Piccoli et al.29
BM48-31/BGR/2008 RBD	This study
Pangolin GX RBD	This study
ZC45 RBD	This study
SARS-CoV RBD	This study
Beta RBD	This study
Delta RBD	This study
Omicron BA.1 RBD	This study
Omicron BA.2 RBD	This study
Omicron BA.3 RBD	This study
Omicron BA.4/5 RBD	This study
Human ACE2	Piccoli et al.29
Blocker™ Casein in PBS	Thermo Fisher Scientific	Cat#37528
Tween 20	Sigma Aldrich	Cat#93773-1KG
Goat Anti-Human IgG-AP	Bioconcept	Cat#2040-04; RRID: AB_2795643
Sodium thiocyanate	Sigma-Aldrich	Cat#251410-2.5KG
Zombie Aqua Fixable Viability Kit	BioLegend	Cat#423101
Ficoll-Paque PLUS (6 x500ml)	VWR International	Cat#17-1440-03
RPMI-1640 W/O L-Glutamine (10x500ml)	Life Technologies Europe BV	Cat#31870074
HyClone Fetal Bovine Serum, US Origin 500ml,	VWR International	Cat#SH30070.03
DPBS w/o Ca and Mg (500ml),	Chemie Brunschwig	Cat#P04-36500 Pan Biotech
MEM NEAA Solution 100x, 100ml,	Bioconcept	Cat#5-13K00-H
Stable Glutamine solution (L-Ala/L-Gln)100x, 100ml	Bioconcept	Cat#5-10K50-H
Penicillin-Streptomycin	Bioconcept	Cat#4-01F00-H
Kanamycin (5,000ug/ml), 100ml	Bioconcept	Cat#4-08F00-H
Transferrin (Holo) from human serum	LuBioscience	Cat#0905-100
2-Mercaptoethanol 50MM	Bioconcept	Cat#5-69F00-E
Sodium Pyruvate (100mM, 100 ml)	Bioconcep	Cat#5-60F00-H
Experimental models: Cell lines
FreeStyleTM 293-F Cells	ThermoFisher Scientific	Cat# R79007; RRID: CVCL_D603
Expi293FTM Cells	ThermoFisher Scientific	Cat# A14527; RRID: CVCL_D615
ExpiCHO-STM	ThermoFisher Scientific	Cat# A29127; RRID: CVCL_5J31
Recombinant DNA
SARS-CoV-2 NTD pCMV plasmid	McCallum et al.13
SARS-CoV-2 S phCMV1 plasmid	Walls et al. and Starr et al.28,47
SARS-CoV S phCMV1 plasmid	Walls et al.48
HCoV-OC43 S phCMV1 plasmid	Pinto et al.49
MERS-CoV S phCMV1 plasmid	Walls et al.48
HCoV-HKU1 S phCMV1 plasmid	Pinto et al.49
SARS-CoV-2 RBD phCMV1 plasmid	Piccoli et al.29
BM48-31/BGR/2008 RBD phCMV1 plasmid	This study
Pangolin GX RBD phCMV1 plasmid	This study
ZC45 RBD phCMV1 plasmid	This study
SARS-CoV RBD phCMV1 plasmid	This study
Beta RBD phCMV1 plasmid	This study
Delta RBD phCMV1 plasmid	This study
Omicron BA.1 RBD phCMV1 plasmid	This study
Omicron BA.2 RBD phCMV1 plasmid	This study
Omicron BA.3 RBD phCMV1 plasmid	This study
Omicron BA.4/5 RBD phCMV1 plasmid	This study
Human ACE2 phCMV1 plasmid	Piccoli et al.29
Software and algorithms
Flowjo (v10.7.1)	FlowJo	https://www.flowjo.com
GraphPad Prism (v9.3.1)	GraphPad	https://www.graphpad.com
Everest (v3.0)	Bio-Rad	https://www.bio-rad.com
Microsoft Excel for Microsoft 365 MSO (Version 2204 Build 16.0.15128.20240)	Microsoft	https://www.microsoft.com