TABLE 2.

Oligonucleotides designed in this study to specifically detect porcine cytokine and cyclophilin cDNA

Gene specificity	Primera	Oligonucleotide sequences (5′–3′)	No. of cycles
IL-1α	S	TGCCAGCTATGAGCCACTTCC	40
	AS	TGACGGGTCTCGAATGATGCT
IL-4	S	TACCAGCAACTTCGTCCAC	45
	AS	ATCGTCTTTAGCCTTTCCAA
IL-6	S	ATGAACTCCCTCTCCACAAGC	45
	AS	TGGCTTTGTCTGGATTCTTTC
IL-8	S	TTTCTGCAGCTCTCTGTGAGG	40
	AS	CTGCTGTTGTTGTTGCTTCTC
IL-12p40	S	GATGCTGGCCAGTACACC	40
	AS	TCCAGCACGACCTCAATG
IFN-γ	S	GTTTTTCTGGCTCTTACTGC	45
	AS	CTTCCGCTTTCTTAGGTTAG
TNF-α	S	GATGGCAGAGAGGAGGTTGAC	38
	AS	ATCGGCCCCCAGAAGGAAGAG
Cyclophilin	S	TAACCCCACCGTCTTCTT	29
	AS	TGCCATCCAACCACTCAG

^aS, sense primer; AS, antisense primer.