Table 1.
Mutation | Mutations | Product observed |
Turnover efficiency (with respect to WT) |
Substrate- bound spin state (390/418 nm), WT at 1.0 |
---|---|---|---|---|
3M | V247A-V29SI-V396I | 5-exo-hydroxy | 39% | 0.45 |
4M | V247G-G248A-V295I-V396I | 5-exo-hydroxy | 13 % | 0.2 |
2S | F87Y-Y96F | 5-exo-hydroxy | 60 % | 0.51 |
4M2S | F87Y-Y96F-V247G-G248A-V295I-V396I | 5-exo-hydroxy (traces) | <1 % | 0.1 |