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. Author manuscript; available in PMC: 2023 Aug 6.
Published in final edited form as: Biochemistry. 2022 Aug 12;61(17):1790–1800. doi: 10.1021/acs.biochem.2c00233

Table 1.

In vitro efficiencies and spin state equilibria of mutants in the turnover of substrate (d-camphor) relative to WT based on comparative substrate/product peak areas in GC analysis of extracts. See Supplementary Material for details.

Mutation Mutations Product
observed
Turnover
efficiency
(with respect
to WT)
Substrate-
bound
spin state
(390/418
nm), WT
at 1.0
3M V247A-V29SI-V396I 5-exo-hydroxy 39% 0.45
4M V247G-G248A-V295I-V396I 5-exo-hydroxy 13 % 0.2
2S F87Y-Y96F 5-exo-hydroxy 60 % 0.51
4M2S F87Y-Y96F-V247G-G248A-V295I-V396I 5-exo-hydroxy (traces) <1 % 0.1