FIG. 4.

Transmission electron microscopy and immunofluorescence microscopy analysis of the cellular localization of trypanosome HSP60. (a and b) Indirect immunogold labeling reveals the presence of HSP60 in the mitochondrion (m) and around the kinetoplast (k) (ma, maculae adherentes of the junction complex between the body and the flagellum). Bar, 0.5 μm. (c to e) Immunofluorescence shows the colocalization of HSP60 (FITC staining) (c and e) with the mitochondrial marker ATP synthase (Texas red staining) (d and e) in the mitochondrion. The DAPI blue fluorescence was used to indicate the nucleus (solid arrows) and the kinetoplast (arrowheads). Bar, 10 μm.