FIG. 8.

ELISA detection of IgG2a anti-HSP60 antibody isotype responses during experimental pleomorphic infection of BALB/c mice with T. brucei AnTat 1.1E parasites. The screening was performed on synthetic trypanosome HSP60 peptides (pep 1, pep 2) (a) and synthetic mouse HSP60 peptides (pep 1, pep 2) (5 μg/ml) (b) with serial dilutions of serum collected at various time points of infection; the starting serum dilution was 1/25. Measurements were made with the sera collected from 5 individual mice, and the values represents the means of specific end-point serum titers and standard deviation, calculated with noninfected serum as a background detection limit.