Figure 2.

Characterization of transcript isoform diversity in HUVECs via long-read RNA-Seq. (A) Schematic of the long-read RNA-seq analysis pipeline. (B) Transcripts and genes identified from PacBio long-read RNA-seq. The number of known (blue) and novel isoforms (green and orange) are shown. (C) Transcript abundance distribution for known (FSM) versus novel transcripts (NIC, NNC), with dashed lines representing median abundance values in full-length read counts per million (CPM) for each category (FSM = 2.4, NIC = 1.5, NIC = 1.3). (D) Distribution of the number of genes expressing multiple isoforms. (E) Fraction of genes in which the most abundantly expressed isoform (“major isoform”) differs from the reference isoform (APPRIS principal isoform).