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. 2022 Dec 1;11(1):2146860. doi: 10.1080/2162402X.2022.2146860

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© 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — CAF and TAM inhibit the cytotoxicity of NK cells against NB cells by a TGF-β1-dependent mechanism.

a. Schema representing the design of the experiment as described in Materials and Methods. b. Top: Direct cytotoxicity against NB cells (CHLA-255-GFP, CHLA-136-GFP) in the presence of naive aNK cells or aNK cells pre-incubated in the presence of conditioned MSC and MN and NB cells (triple trained aNK). The analysis was performed by IncuCyte as indicated in Materials and Methods. The data represent the percent of live cells over 24 h and are representative of one from three independent experiments. Bottom: Bar graphs representing the mean ±SD percent of live cells after 8 h of culture from three independent experiments done in technical duplicates. c. Top: aNK cells were treated for 72 h with the indicated concentrations of rTGF-β1 (0.1–5 ng/mL), and their cytotoxic activity against NB cells (CHLA-255-GFP, CHLA-136-GFP) was determined by IncuCyte as in panel B. The data represent the percent of live cells over 24 h and are representative of one from three independent experiments. Bottom: Bar graphs representing the mean ±SD percent of live cells after 8 h of culture from three independent experiments done in technical duplicates. The p-values were determined by Wilcoxon–Mann–Whitney test. ****p < .0001, ***p < .001, **p < .01 ns: not significant.