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. 2022 Dec 2;15(1):2149055. doi: 10.1080/19420862.2022.2149055

Figure 2.

Part A shows two column graphs of the number of rearing events. The graph on the left shows that the number of rearing events significantly decreased in AIA mice treated with vehicle and significantly increased in AIA mice treated with 0.2 and 0.6 mg/kg anti-NGF Fab’-PEG. The graph on the right shows that the number of rearing events significantly decreased in AIA mice treated with vehicle and significantly increased in AIA mice treated with 0.3 mg/kg anti-NGF IgG. Part B shows biofluorescence images of the paws of 4 animals in the anti-NGF Fab’-PEG-treated group in the top row and the anti-NGF IgG-treated group on the bottom row. The intensity of the biofluorescence signal was higher in anti-NGF Fab’-PEG-treated animals than in anti-NGF IgG-treated animals. Part C shows a time plot of fluorescence signal in CIA or normal mice treated with anti-NGF Fab’-PEG or anti-NGF IgG up to 50 hours after administration. Part D shows a column graph of the fluorescence measured 2 hours after administration. Anti-NGF IgG-treated CIA mice show a significant increase in fluorescence compared to anti-NGF IgG-treated normal mice, and anti-NGF Fab’-PEG-treated CIA mice show a significant increase in fluorescence compared to anti-NGF IgG-treated CIA mice.

Analgesic effect and distribution of anti-NGF Fab’-PEG in arthritis models. (a) Analgesic effect of the anti-NGF Fab’-PEG and anti-NGF IgG in a murine AIA model. The number of rearing events was measured as an indicator of pain. Data are expressed as the mean ± SEM of 7–8 mice in each group. ### P < .001, statistically significant compared to the sham group (Student’s t-test). * P < .05, ** P < .01, *** P < .001, statistically significant compared to the vehicle group (Dunnett’s multiple comparisons test). (B, C, D) Biofluorescence imaging of the anti-NGF Fab’-PEG and anti-NGF IgG distribution in the inflamed paws of the murine CIA model. The images were captured and analyzed using the IVIS Spectrum in vivo imaging system (B, 2 hours after administration in CIA mice) and the fluorescence intensities were plotted at each time point. Change in fluorescence intensity (c) and fluorescence intensity 2 hours after administration (d). Data are expressed as the mean ± SEM of 4 mice in each group. ## P < .01, statistically significant compared to the anti-NGF IgG-Normal group. ** P < .01, statistically significant compared to the anti-NGF IgG-CIA group (Student’s t-test).