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. Author manuscript; available in PMC: 2022 Dec 5.
Published in final edited form as: Nature. 2021 Dec 8;600(7889):494–499. doi: 10.1038/s41586-021-04183-x

Extended Data Fig. 2 |. Changes in behavioural and hippocampal biological processes in response to runner plasma infusions.

Extended Data Fig. 2 |

a, Plasma from running mice (3–4 months of age) was collected and transferred to matched aged non-running mice, once every 3 days for 28 days. Mice were then tested for cued memory on the fear conditioning test. Graphs show percentage of freezing behaviour in response to the light/tone cues associated with the fear stimulus in CP and RP recipient mice (n = 18–20 per group). Means ± s.e.m; Unpaired Student’s two-tailed t test, N.S. not significant. b, CP and RP infused mice show comparable activity and velocity in the activity chamber (n = 13–14 per group) and percentage of time spent in the light arena (n = 12 per group). Means ± s.e.m; unpaired Student’s two-tailed t test; N.S., not significant. c, Graphs show distance traveled, thigmotaxis and velocity displayed by mice infused with RP or CP in the water maze test (n = 12 per group). d, Hierarchical networks of the abundance of gene ontology (GO) terms (Fisher’s exact test, P < 0.05) related to biological processes using REVIGO (Resnik measurement, 0.7 distance). GO terms correspond to the DEGs with treatment of CP versus RP shown in Fig. 1h.