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. 2022 Dec 5;20(12):e3001730. doi: 10.1371/journal.pbio.3001730

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© 2022 Huang et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — (A) Direct curve-fitting comparisons between PreScale during early aging from 1d to 30d (Fig 1) and genetic brp titration from 1xBRP to 4xBRP at age 5d [33]. (B and C) Lifespan analysis of flies with BRP titration from 1xBRP to 4xBRP. For male flies (B), n = 394 for 1xBRP (p < 0.001), n = 396 for 3xBRP (p < 0.001) and n = 395 for 4xBRP (p < 0.001), compared to 2xBRP wt (n = 393). For female flies (C), n = 400 for 1xBRP (p = 0.017), n = 399 for 3xBRP (ns) and n = 397 for 4xBRP (p < 0.001), compared to 2xBRP wt (n = 399). (D and E) An independent experiment of the lifespan of 2xBRP and 3xBRP flies. For male flies (D), n = 94 for 3xBRP compared to 2xBRP wt (n = 36, p < 0.001). For female flies (E), n = 98 for 3xBRP compared to 2xBRP wt (n = 59, p < 0.001). (F and G) Lifespan analysis of flies with 3xBRP in wake mutant background. For male flies (F), n = 234 for 2xBRP;wake compared to 2xBRP wt control (n = 233, p < 0.001) or compared to 3xBRP,wake (n = 155, p < 0.001). For female flies (G), n = 235 for 2xBRP;wake compared to 2xBRP wt control (n = 230, p < 0.001) or compared to 3xBRP,wake (n = 231, ns). (H and I) Lifespan analysis of flies with 3xBRP in inc mutant background. For male flies (H), n = 236 for inc;2xBRP compared to 2xBRP wt (n = 232, p = 0.009) or compared to inc;3xBRP (n = 212, p < 0.001). For female flies (I), n = 235 for inc;2xBRP compared to 2xBRP wt (n = 235, p < 0.001, wt is also shown in Fig 1A) or compared to inc;3xBRP (n = 182, ns). (J and K) Lifespan analysis of flies with 3xBRP in atg7 mutant background. For male flies (J), n = 108 for atg7,2xBRP compared to 2xBRP wt (n = 195, p < 0.001) or compared to atg7;3xBRP (n = 87, ns). For female flies (K), n = 109 for atg7,2xBRP compared to 2xBRP wt (n = 208, p < 0.001) or compared to atg7;3xBRP (n = 87, p < 0.001). (L and M) Lifespan analysis of flies with 3xBRP in Alzheimer’s disease model flies (elav>Aβ). For male flies (L), n = 203 for 2xBRP;Aβ compared to elav>mCD8-GFP control (n = 238, p < 0.001) or compared to 3xBRP;Aβ (n = 151, p = 0.006). For female flies (M), n = 249 for 2xBRP;Aβ compared to elav>mCD8-GFP control (n = 228, p < 0.001) or compared to 3xBRP;Aβ (n = 241, p = 0.002). Gehan–Breslow–Wilcoxon test with Bonferroni correction for multiple comparisons is shown for all longevity experiments. (N and O) STM for 5d (N) and 30d (O) 2xBRP and 3xBRP flies. n = 19 for 5d 2xBRP, n = 12 for 5d 3xBRP. n = 7–8 for 30d for both groups at 30d. (P and Q) MTM tested 3 h after training for 5d (P) and 30d (Q) 2xBRP and 3xBRP flies. n = 21 for 5d 2xBRP, n = 16 for 5d 3xBRP. n = 8–9 for 30d for both groups at 30d. Student t test is shown. *p < 0.05; ns, not significant. Error bars: mean ± SEM. Underlying data can be found in S3 Data. BRP, Bruchpilot; MTM, middle-term memory; STM, short-term memory; wt, wild type.