FIG. 1.

Detection of apoptosis in M. ulcerans-infected guinea pig lesions and media controls. The TUNEL reaction was performed, detected by use of DAB, and counterstained with hematoxylin as described in Materials and Methods. A to C, negative control section injected with mycobacterial medium; D to F, M. ulcerans-injected section (8 days postinjection). A and D, ×83; B, C, E, and F, ×332. Panels A, B, D, and E are hematoxylin-eosin-stained sections; panels C and F are TUNEL sections. Arrows point to the blood vessels; muscle cells (m) are also indicated.