FIG. 2.

Transcytosis times of WT MS11A and the MS11A fitA mutant. Polarized T84 monolayers were infected with WT or fitA bacteria, both P⁺ Opa⁻. At the indicated times, the presence of bacteria in the basal medium (i.e., exocytosed bacteria) was determined as described in Materials and Methods. The results are the sum of two independent experiments. A total of 16 polarized monolayers were infected with WT bacteria (white bars), and 18 monolayers were infected with fitA bacteria (black bars).