FIG. 1.

2D map (pH 4 to 8) of a whole-cell lysate of H. pylori G27 and identification of H. pylori antigens by immunoblot analysis. A 100-μg portion of a whole-cell lysate of H. pylori G27 was loaded onto the IEF gels. Identified proteins are indicated by the spot numbers given in Table 1. The positions of molecular weight (MW) standards are indicated on the right. (A) Silver stain of a typical 2D gel. (B) Western blot of a duplicate 2D gel hybridized with serum AR, which is derived from an H. pylori-infected individual suffering from gastritis. Western blots were developed using the enhanced chemiluminescence detection system (Amersham). (C) Western blot of a duplicate 2D gel hybridized with a control serum from an H. pylori-negative individual (N1).