TABLE 1.

Identification of immunogenic H. pylori proteins by immunoblotting and LC-MS^a

Spot no.	Antigen	HP no.	MW (103)	pI
1	Neutrophil-activating protein (NapA) (bacterioferritin)	HP0243	16	5.9
2	Cochaperone HspA (GroES)	HP0011	13	6.6
3	Alkyl hydroperoxide reductase	HP1563	22	6.3
4	DNA-directed RNA polymerase, α subunit	HP1293	38	4.8
5	Isocitrate dehydrogenase	HP0027	47	7.9
6	Conserved hypothetical protein	HP0318	28	7.7
7	Translation elongation factor EF-P	HP0177	20	5.4
8	RecA protein	HP0153	38	5.8
9	Translation elongation factor EF-Ts	HP1555	39	6.5
10	Pyruvate ferredoxin oxidoreductase, α subunit	HP1110	45	5.8
11	Aspartate ammonia-lyase	HP0649	51	6.8
12	Flagellin A (FlaA)	HP0601	53	6.3
13	70-kDa chaperone (DnaK)	HP0109	68	4.8
14	Trigger factor	HP0795	49	5.2
15	Flagellar hook-associated protein 2 (FliD)	HP0752	74	5.1
16	Urease B subunit (UreB)	HP0072	63	5.9
17	Peptidoglycan-associated lipoprotein precursor (Omp18)	HP1125	20	5.3
18	Translation elongation factor EF-Tu	HP1205	44	5.0
19	Chaperone and heat shock protein HspB (GroEL)	HP0010	60	5.6
20	Ribosomal protein L7/L12	HP1199	14	5.0
21	ATP synthase F1, subunit β	HP1132	52	5.2
22	Biotin carboxyl carrier protein	HP0371	17	5.4
23	Hydrogenase expression/formation protein	HP0900	27	5.3
24	Glutamine synthetase	HP0512	53	6.1
25	Hemolysin secretion protein precursor	HP0599	48	6.2
26	Hypothetical protein	HP1037	39	6.1
27	3-oxoadipate coenzyme A-transferase subunit A	HP0691	25	5.9
28	Aconitase B	HP0779	94	6.5
29	ATP-dependent protease binding subunit (ClpB)	HP0264	94	6.3

^aTheoretical molecular weight and isoelectric point were calculated from the ORFs annotated in the genomic sequence of H. pylori 26695 (33). Note that ORF products corresponding to HP0599 and HP1037 were annotated as methyl-accepting chemotaxis protein (JHP546; 97% pairwise protein identity to HP0599) and proline peptidase (JHP387; 96.4% pairwise protein identity to HP1037) in the genomic sequence of the unrelated H. pylori strain J99 (2).