Figure 4.

Transient transfection of mimics that target 3′UTR of NHE-3 did not alter mRNA expression in SK-CO15 Cells. RNA was extracted from SK-CO15 cells 48 h after transfection with microRNAs mimics that target 3′UTR of NHE-3. NHE-3 mRNA levels were measured by quantitative real-time PCR and were normalized against GAPDH mRNA levels. Results are means ± SE of four independent experiments. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; NHE-3, sodium hydrogen exchanger-3; 3′UTR, 3′ untranslated region.