Figure 5.

Protein expression of NHE-3 was decreased by mimic-326 and mimic-330-5p transfection in SK-CO15 cells. A: Western blot analysis showing blot probed with anti-NHE-3 or anti-GAPDH antibody from 48 h mimic transfected lysates. B: densitometric analysis of the relative band intensities NHE-3/GAPDH using imageJ. Results represent means ± SE of four independent experiments. **Significant differences between control negative vs. mimic-326 (P < 0.001) and * vs. mimc-330-5p transfected groups (P < 0.05). GAPDH, glyceraldehyde 3-phosphate dehydrogenase; NHE-3, sodium hydrogen exchanger-3; 3′UTR, 3′ untranslated region.