Table 1.

Primers used in the study

Primers used for cloning 3´UTR-NHE-3 in pmirGLO vector:

Forward: 5′-ACTGCTAGCCACCGGCTCCGACACGCCGCTAAC-3′

Reverse: 5′-ACTCTCGAGGGAGTTCTGCGCAGGCGCTGGCGT-3′

Real time PCR primers:

NHE-3:

Forward: 5′-ACCTGTTCGTCAGCACCAC-3′.

Reverse: 5′-GCTCGCTCCTCTTCACCTT-3′

GAPDH:

Forward: 5′-GAAATCCCATCACCATCTTCC-3′.

Reverse: 5′-AAATGAGCCCCAGCCTTCT-3′

Forward primers used for quantifying microRNA expression:

hsa-miR-204: 5′-TTCCCTTTGTCATCCTATGCCT-3′

hsa-miR-326: 5′-CCTCTGGGCCCTTCCTCCAG-3′

hsa-miR-330-5P: 5′-TCTCTGGGCCTGTGTCTTAGGC-3′

hsa-miR-744-5p: 5′-TGCGGGGCTAGGGCTAACAGCA-3′

U6B: 5′-CGCAAGGATGACACGCAAATTCG-3′

A common universal reverse primer provided in the kit was used

NHE-3, sodium hydrogen exchanger-3; 3′UTR, 3′ untranslated region.