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. 2022 Oct 25;323(6):R921–R934. doi: 10.1152/ajpregu.00213.2022

Figure 1.

Figure 1.

Representative interleukin 11 receptor, α chain 1 (Il11ra1) amplicons generated from the progeny of IL-11Rα1+/− breeding pairs. As described in materials and methods, genomic DNA was extracted from tails snips obtained from wild-type mice, mice heterozygous for a null mutation in Il11ra1 (IL-11Rα1+/− mice), and mice homozygous for a null mutation in Il11ra1 (IL-11Rα1-deficient mice) and amplified via PCR using specific primers for the Il11ra1 wild-type and mutant alleles. PCR products were resolved on a 1.8% ethidium bromide-stained agarose gel. Amplification of genomic DNA via PCR from 1) wild-type mice generated one amplicon of 766 base pairs (bp), 2) IL-11Rα1+/− mice generated two amplicons of 766 and 593 bp, and 3) IL-11Rα1-deficient mice generated one amplicon of 593 bp.