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. 2022 Nov 28;2022:8537881. doi: 10.1155/2022/8537881

Figure 1.

Figure 1

Resveratrol inhibits insulin-induced VSMC proliferation. (a) Effect of insulin on VSMC proliferation. After incubating VSMCs with various insulin concentrations for 24 h, CCK-8 assay was performed to assess the OD values at 450  nm for each treatment group. ∗P < 0.05 vs. control, n = 5/group. (b) Effect of resveratrol on VSMC proliferation. A10 cells were treated with different concentrations of resveratrol (25-200 µM) for 24 h. Cell proliferation was quantified using the CCK-8 method and compared with that of the control group. ∗P < 0.05 vs. control, n = 5/group. (c) and (d) effect of resveratrol on insulin-induced proliferation of VSMCs. Cell proliferation was detected using the CCK-8 assay (c) or direct cell counting (d) after incubation with insulin (100 nM) in the absence or presence of resveratrol (25-200 μM). ∗P < 0.05 vs. control; #P < 0.05 vs. insulin group, n = 6/group. (e) and (f) EdU incorporation assay was performed to detect the effect of resveratrol (100 μM) on VSMC proliferation after pretreatment with insulin (100 nM). (e) DNA synthesis in VSMCs was tested by EdU incorporation. (f) The ratio of EdU-positive cells to total cells. ∗P < 0.05 vs. control; #P < 0.05 vs. insulin group, n = 9/group.