Figure 4.

Role of resveratrol in the effect of insulin-induced PI3K/Akt activation (a) and (b) effect of insulin on PI3K signaling pathway in VSMCs. A10 cells were incubated with insulin (100 nM) for 30 min, and Western blotting was performed to analyze PI3K and Akt phosphorylation levels. Relative phosphorylation levels of PI3K and Akt were quantified. ^∗P < 0.05 vs. control, n = 5/group. (c) and (d) Effect of resveratrol on insulin-induced PI3K/Akt activation in VSMCs. Western blotting identified the protein expression levels of p-PI3K and p-Akt, whether or not resveratrol 100 μM was pretreated. Bar graph showing the relative protein expression of phosphorylated and total PI3K and Akt. ^∗P < 0.05 vs. control; #P < 0.05 vs. insulin group, n = 5/group.