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. 2022 Nov 16;46(1):5–13. doi: 10.1097/CJI.0000000000000445

FIGURE 3.

FIGURE 3

10:1 aAPC:γδ T cell ratio is optimal for expansion. A, Experimental timeline. PBMCs were cultured with zol and IL-2 at day -7. Zol enriched γδ T cells were cocultured with irradiated aAPCs at 1:0, 1:1, 5:1, 10:1, and 50:1 aAPC:γδ T cell ratios. A portion of cells was collected at days 7 and 10 for enumeration and phenotyping by flow cytometry. B, γδ T cell fold change and cell counts are highest at a 10:1 aAPC:γδ T cell ratio at days 7 and 10. C, CD16+ γδ T cell fold change and counts are similar at either a 10:1 or 50:1 aAPC:γδ T cell ratio. D, CD56+ γδ T cells have the highest fold change and count at a 10:1 aAPC:γδ T cell ratio at days 7 and 10. Data is from 1 healthy donor.