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. 2022 Nov 16;46(1):5–13. doi: 10.1097/CJI.0000000000000445

FIGURE 4.

FIGURE 4

The coculture of zol enriched γδ T cells with K-562 aAPCs enhances expansion and memory phenotype. A, Flow cytometry gating strategy for γδ T cells. At days 7 and 10, a portion of cells was removed, counted, and phenotypic markers were analyzed by flow cytometry. B, Coculture of γδ T cells with aAPCs results in expansion. C, γδ T cell absolute numbers increase with aAPC coculture. D, CD16+ γδ T fold change increases after 10 days of aAPC coculture. E, CD56+ γδ T fold change expands with aAPC coculture. Percentage of γδ T cells that are PD1+ (F), LAG3+ (G), or TIM3+ (H). I, NKG2D percentage, fold change, and the number of γδ T cells expressing NKG2D increases after 10 days of aAPC coculture. J, Percentages of γδ T cell memory phenotypes at indicated days. At day 0 γδ T cells were added to irradiated aAPCs at a 10:1 aAPC:γδ T cell ratio. Data shows 3 independent, healthy donors.