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Knockout of nc886 in FaDu cells is lethal
Nc886 gene was replaced by GFP using CRISPR technology. (A) Sanger sequencing of nc886 gene in nc886KO cells showing replacement by GFP gene. (B) Representative images of two nc886KO colonies after moving from 96-well plates to 6-well plates. All the selected GFP(+) cell colonies died during expansion.
