REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-CD45-Pacific Blue	BioLegend	Cat#103126; RRID:AB_493535
Anti-CD31-Pacific Blue	BioLegend	Cat#102422; RRID:AB_10612926
Anti-TER-119-Pacific Blue	BioLegend	Cat#116232; RRID:AB_2251160
Anti-CD24-APC	eBioscience	Cat#17-0242-82; RRID:AB_10870773
Anti-ICAM1-PE-CY7	BioLegend	Cat#116122; RRID:AB_2715950
Anti-NCAM-PE	R&D Systems	Cat#FAB7820P; AB_2924964
Anti-DLL3	Invitrogen	Cat#PA5-23448; RRID:AB_2540948
Anti-HES1	Cell Signaling Technology	Cat#11988; RRID:AB_2728766
Anti-hexahistidine	Cell Signaling Technology	Cat#12698S; RRID:AB_2744546
Anti-Rabbit-AF488	Invitrogen	Cat#A11008; RRID:AB_143165
Bacterial and virus strains
BL21 (DE3) electrocompetent cells	SigmaAldrich	CMC0016
Endura competent cells	Lucigen	60240-2
Chemicals, peptides, and recombinant proteins
RPMI-1640	Millipore Sigma	R0883
DMEM High-Glucose medium	Hyclone	SH30243.01
Bovine Growth Serum	Hyclone	SH3054103HI
Penicillin-streptomycin-glutamine	Gibco	10378016
Ampicillin sodium salt	SigmaAldrich	A0166
IPTG	Gold Biotechnology	I2481C5
B-PER	Thermo Fisher Scientific	90084
Lysozyme	SigmaAldrich	L4919
DNase I	Roche	10104159001
Ni-NTA agarose	Qiagen	30210
Centrifugal filters	Millipore	UFC801024
AF680 succinimidyl ester	Thermo Fisher Scientific	A20008
Albumin from bovine serum	SigmaAldrich	A9647-100G
Polyethylenimine	Polysciences	23966-1
Histo-Clear	National Diagnostics	HS-200
Antigen unmasking solution	Vector Laboratories	H-3300
Hematoxylin	SigmaAldrich	HHS32-1L
L-15 medium	Sigma	L1518
Collagenase I	Sigma	C0130
Collagenase II	Sigma	6885
Collagenase IV	Sigma	5138
Elastase	Worthington	LS002292
RBC lysis buffer	eBioscience	00-4333-57
Critical commercial assays
mmPRESS®HRP Horse Anti-Rabbit IgG Polymer Detection Kit	Vector Laboratories	MP-7401
ImmPRESS® Excel Amplified Polymer Staining Anti-Rabbit IgG Peroxidase Kit	Vector Laboratories	MP-7601
DAB substrate kit	Vector Laboratories	SK-4100
Mouse Direct PCR kit	Bimake	B40013
Deposited data
MATLAB code for modeling	GitHub	https://github.com/junwkim1/DLL3_lateral_inhibition_2022
Experimental models: Cell lines
NCI-H82	ATCC	NCI-H82
NCI-H146	ATCC	NCI-H146
NCI-H524	ATCC	NCI-H524
HEK-293T	ATCC	293T
KP11B6	Julien Sage Laboratory	Park et al., 201163
HES1pos, HES1neg cells	Julien Sage Laboratory	Lim et al., 201712
Experimental models: Organisms/strains
Mouse: NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ	The Jackson Laboratory	Cat#005557
RPR2 model	Julien Sage Laboratory	Park et al., 201163
Rosa26LSL-Dll3	GenOway	genOway/MNO/ABB55-Rosa26_Dll3
Rosa26LSL-Luc	Julien Sage Laboratory	Jahchan et al., 201645
Oligonucleotides
See Table S2
Recombinant DNA
pCDH-CMV-MCS-EF1-puro	System Biosciences	CD510B-1
Software and algorithms
GraphPad Prism	GraphPad	https://www.graphpad.com/
FlowJo	FlowJo	https://www.flowjo.com/
MATLAB	MathWorks	https://www.mathworks.com/
BZ-X Viewer 1.3.1.1	Keyence	https://www.keyence.com/products/microscope/fluorescence-microscope/bz-x700/
BZ-X Analyzer 1.4.0.1	Keyence	https://www.keyence.com/products/microscope/fluorescence-microscope/bz-x700/