Fig. 7.

Experimental protocol for AAV-mediated SQOR gene transfer.

Mice on post-natal day 2 received intracerebroventricular (ICV) injection of AAV9-hSYN1-mSQRDL-hSYN1-eGFP (AAV-SQR) or AAV9-hSYN1-eGFP (AAV-GFP) at 10¹⁰ viral particles per hemisphere. After 24 weeks, mice received four intraperitoneal injections of either MPTP (20 mg/kg) or saline. For each animal, there was a 2 h interval between each IP injection. The nigrostriatal regions were harvested 7 days after MPTP administration and the levels of tyrosine hydroxylase were determined using immunoblot.